925dad7c-7d60-4bff-a4f9-dca031d3d396 Laura Zucconi University of Tuscia zucconi@unitus.it 2026-08-31T12:58:15 ISO 19139 1.0 WGS84 DNA metabarcoding analysis of bacterial communities in rocks and soils of Northern Victoria Land - AMICO - 2022 2021-10-01 https://antarcticdatacenter.cnr.it/geonetwork/srv/api/records/fb76df45-1654-46c0-b827-b4252769fe0f The bacterial communities in rocks and soils of three different localities (Battleship Promontory, Richard Nunatak, Trio Nunatak), where stations are collecting climatic data, have been compared with a DNA metabarcoding approach. In addition samples have been trated with Propidium Mono-Azide (PMA) which binds to DNA of damaged cells and inhibit its replication via PCR. The comparison between samples treated with PMA and samples not treated allows to check the bacterial cells that are intact and potentially alive in these substrates. The results showed a strong differentiation in the communities diversity and composition between the two substrates, with an higher diversity in soils compared to rocks. In addition, the PMA treatment revealed the presence of an high proportion of death cells, whose amplification in traditional metabarcoding approaches obscures the correlation trends in this environment. Laura Zucconi University of Tuscia zucconi@unitus.it Fabiana Canini canini.fabiana@unitus.it https://antarcticdatacenter.cnr.it/geonetwork/srv/api/records/925dad7c-7d60-4bff-a4f9-dca031d3d396/attachments/immagine.jpg Antarctica Richards Nunatak Trio Nunataks Battleship Promontory Victoria Land SCAR Gazetteer of Antarctica 2021-01-01 geonetwork.thesaurus.external.theme.scar_gazetteer_multi Soil Habitats and biotopes Species distribution GEMET - INSPIRE themes, version 1.0 2008-01-01 geonetwork.thesaurus.external.theme.httpinspireeceuropaeutheme-theme SOILS GCMD - Science Keywords 2015-01-01 geonetwork.thesaurus.external.theme.gcmd-sciencekeywords Soil bacteria, rock bacteria, DNA metabarcoding AMICO PNRA0000005 PNRA project 2026-06-17 geonetwork.thesaurus.external.theme.PNRAproject Università della Tuscia Research Organization 2024-03-18 geonetwork.thesaurus.external.theme.Research_Organization IT/PNRA GCMD - Providers 2023-07-24 geonetwork.thesaurus.external.theme.thesaurus Initiative and Programs 2023-10-02 geonetwork.thesaurus.local.theme.InitiativeandPrograms Creative-Commons CC BY 4.0 NUMERO SPEDIZIONE/SPEDIZIONI 1 -180.00 180.00 -90.00 -60.516 2022-01-01 2024-12-31 FASTQ 1 https://www.pnra.aq/it/project/549/antarctic-terrestrial-observation-system-detecting-understanding-and-interpreting WWW:LINK-1.0-http--link PNRA project https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA922885. WWW:LINK-1.0-http--link bacterial dataset in rocks and soils https://antarcticdatacenter.cnr.it/geonetwork/srv/api/records/fb76df45-1654-46c0-b827-b4252769fe0f WWW:LINK-1.0-http--link Resource identifier COMMISSION REGULATION (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 This data set is conformant with the INSPIRE Implementing Rules for the interoperability of spatial data sets and services true Data collected in Antarctica, DNA sequences are store in NCBI data bank.